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Prof. Melinda Sheffield-Moore led the research at University of Texas. She said that a burden of ageing is weakness & muscle wasting. Muscle mass increased by 2-4% in the elderly given small amounts of amino acids, even without additional exercise. She said that with exercise this would strengthen muscles even more. While there isn't necessarily one age where you wake up feeling old, 45 does seem to be an important age when it comes to cardio fitness. In one study published in the Archives of Internal Medicine, researchers studied over 20,000 men and women from 1974 to 2006. They looked at lifestyle variables such as body mass index, self-reported aerobic exercise, and smoking while measuring their cardio fitness by using a treadmill test. Researchers found that cardio fitness declines over time, but goes down hill at a faster rate after 45 years of age, at least for those participants who didn't exercise.